Intracellular delivery of bio-macromolecules including glycosaminoglycans etc.Intracellular delivery of exogenous or recombinant proteins including peptides, enzymes, antibodies (a) 25 g of styrene maleic anhydride co-polymer is added to 250 ml of 1 M NaOH and 0.5 g of antibumping granules in a round-bottom flask that rests on a cork ring in the fume hood.FITC-dextran as positive control, 2 mg in vial (vial B).Polysaccharide can be also transfected.Effect to the function of proteins cab be minimized.High efficiency of delivering proteins to cytoplasm.Proteins and ProteoCarry can be delivered into endosomes by endocytosis pathway and also escape to cytosol by its highly membrane-lytic activity with low cytotoxicity. ProteoCarry can overcome this problem based on a novel and potent pH-dependent endosomal membrane-lytic activity. Highly effective endosomal escape of proteins is the most important subject of protein transfection reagents. But protein-reagent complexes are able to escape endosomes with little efficiency and consequently transported to lysosomes to be degraded. Commonly these reagents interact with protein to form complexes and subsequently protein-reagent complexes are entered into cells via endocytosis pathway. Although many protein transfection reagents including peptide-, cationic lipid- and polymer-based compounds have been developed to date, these reagents struggle to deliver proteins into “cytosol”. While plasmid-based gene expression generally needs 12-24 hours to express proteins well, protein transfection reagents can immediately import functional proteins into living cells within several hours. Protein transfection, intracellular delivery of proteins, is a powerful method to analyze cellular response to the protein of interest. ProteoPrep is a registered trademark of Sigma-Aldrich Co.ProteoCarry is a novel peptide-based transfection reagent for exogenous proteins such as functional proteins, peptides and antibodies with highly efficiency of cytosolic delivery. Pre-measured reducing & alkylating reagents - Easy-to-use reagents provide improved IEF resolution. Two pre-mixed solubilization solutions - Removes interfereing non-membrane proteins prior to extractions, resulting in uncluttered 2D arrays. Innovative detergent preparations - Improved solubility allows for higher protein loads and greater visibility of low abundance proteins in 2D gels. This system uses an asymmetric polysulfone membrane to separate plasma from red blood cells, and works with samples as small as 10 microliters. PMID: 8739001 Abstract The authors describe a prototype membrane-based, dry-reagent prothrombin time assay for whole blood. The final protein solution are suitable for 2D gel electrophoresis. 1 Avocet Medical, Inc., Los Gatos, CA 95032, USA. ProteoPrep ® Membrane Extraction Kit is designed to prepare highly enriched membrane protein solutions from many types of cells. Extraction reagents 3 and 4 are ideal for protein extraction prior to isoelectric focusing and 2D electrophoresis. They polymerize at 37 C to form reconstituted basement membranes that are rich in extracellular matrix proteins, such as laminin, collagen IV, entactin, and. Cultrex Basement Membrane Extracts are solubilized extracellular matrices purified from Englebreth-Holm-Swarm (EHS) tumor. Protein Extraction Reagent Types 3 and 4 are optimized for proteomic studies. Cultrex Basement Membrane Extracts for 2-D and 3-D Cell Culture. ProteoPrep ® Membrane Extraction Kit reagents utilize a powerful detergents for higher loading and resolution of proteins in 2D gels.
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